Illumina Single-Cell 3′ RNA Prep<\/a>\u00a0(Illumina Single Cell Prep) \u8bd5\u5242\u76d2\u5df2\u66f4\u65b0\u4e3a\u56e0\u7f8e\u7eb3\u54c1\u724c\u4ea7\u54c1\u5e76\u5728\u5168\u7403\u8303\u56f4\u5185\u4e0a\u5e02\u3002<\/p>\n\u56e0\u7f8e\u7eb3\u516c\u53f8\u9996\u5e2d\u6280\u672f\u5b98Steve Barnard\u535a\u58eb\u8868\u793a\uff1a”NovaSeq X\u4ea7\u54c1\u7cfb\u5217\u662f\u5907\u53d7\u79d1\u5b66\u754c\u4fe1\u8d56\u7684\u65b0\u4e00\u4ee3\u6d4b\u5e8f\u4ea7\u54c1\u3002\u73b0\u5728\uff0c\u6211\u4eec\u914d\u5907\u4e86\u5347\u7ea7\u7248\u8f6f\u4ef6\u548c\u5168\u65b0\u8bd5\u5242\u76d2\uff0c\u5e76\u5e26\u6765\u4e86\u66f4\u4f4e\u901a\u91cf\u7684\u4ea7\u54c1\u9009\u62e9\u3002\u57fa\u4e8e\u5ba2\u6237\u7684\u53cd\u9988\uff0c\u6211\u4eec\u6301\u7eed\u52a0\u5927\u521b\u65b0\u6295\u5165\uff0c\u6253\u9020\u66f4\u52a0\u5f3a\u5927\u4e14\u5e94\u7528\u5e7f\u6cdb\u7684\u4ea7\u54c1\u7cfb\u5217\uff0c\u4ee5\u652f\u6301\u5305\u62ec\u57fa\u56e0\u7ec4\u5b66\u548c\u591a\u7ec4\u5b66\u5728\u5185\u7684\u5b8c\u6574\u5de5\u4f5c\u6d41\u53ca\u66f4\u591a\u7075\u6d3b\u5e94\u7528\u3002\u76ee\u524d\uff0c\u5ba2\u6237\u4eec\u5bf9\u8f6f\u4ef6\u5347\u7ea7\u7684\u5f3a\u5927\u529f\u80fd\u8d5e\u4e0d\u7edd\u53e3\u3002”<\/p>\n
\u5ba2\u6237\u8ba4\u53ef\u8f6f\u4ef6\u5347\u7ea7\u4ef7\u503c<\/p>\n
\u6700\u65b0\u7684\u8f6f\u4ef6\u5347\u7ea7\u5728\u4f18\u5316\u6d4b\u5e8f\u5de5\u4f5c\u6d41\u7a0b\u4e2d\u53d1\u6325\u7740\u5173\u952e\u4f5c\u7528\u30021.3 \u7248\u672c\u8f6f\u4ef6\u4e3a\u5b9e\u9a8c\u5ba4\u63d0\u4f9b\u4e86\u66f4\u9ad8\u7684\u6d4b\u5e8f\u7cbe\u5ea6\u548c\u6570\u636e\u4ea7\u91cf\uff0c\u4ee5\u786e\u4fdd\u53ef\u9760\u3001\u7cbe\u786e\u7684\u6d4b\u5e8f\u7ed3\u679c\uff0c\u5373\u4f7f\u5bf9\u4e8e\u590d\u6742\u3001\u4f4e\u591a\u6837\u6027\u7684\u6837\u672c\u4e5f\u540c\u6837\u9002\u7528\u3002\u672c\u6b21\u5347\u7ea7\u8fd8\u51cf\u5c11\u4e86\u5bf9PhiX\u7684\u9700\u6c42\uff0c\u8fd9\u610f\u5473\u7740\u6bcf\u6b21\u8fd0\u884c\u5c06\u83b7\u5f97\u66f4\u591a\u53ef\u7528\u6570\u636e\uff0c\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u5c3d\u53ef\u80fd\u63d0\u9ad8\u6d4b\u5e8f\u901a\u91cf\uff0c\u589e\u5f3a\u5bf9\u6d4b\u5e8f\u7ed3\u679c\u7684\u4fe1\u5fc3\u3002<\/p>\n
\u9b41\u5317\u514b\u57fa\u56e0\u7ec4\u7814\u7a76\u6240\u662f\u4e00\u5bb6\u975e\u8425\u5229\u7ec4\u7ec7\uff0c\u4e3a45\u4e2a\u56fd\u5bb6\u6216\u5730\u533a\u7684\u5b66\u672f\u548c\u4ea7\u4e1a\u9886\u57df\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u57fa\u56e0\u7ec4\u670d\u52a1\u3002\u5728\u5bf91.3\u7248\u672c\u8f6f\u4ef6\u7684\u6d4b\u8bd5\u4e2d\uff0c\u8be5\u673a\u6784\u53d1\u73b0\u5404\u79cd\u6587\u5e93\u7c7b\u578b\u7684\u6570\u636e\u8d28\u91cf\u5747\u5f97\u5230\u4e86\u663e\u8457\u63d0\u5347\u3002\u9b41\u5317\u514b\u57fa\u56e0\u7ec4\u7814\u7a76\u6240NGS\u6d4b\u5e8f\u56e2\u961f\u8d1f\u8d23\u4ebaGenevi\u00e8ve DonPierre\u8868\u793a\uff1a”\u6570\u636e\u4ea7\u91cf\u589e\u52a0\u3001\u6d4b\u5e8f\u51c6\u786e\u6027\u63d0\u5347\u3001PhiX\u9700\u6c42\u91cf\u964d\u4f4e\uff0c\u4ee5\u53ca\u4f4e\u591a\u6837\u6027\u6587\u5e93\u7684\u4f18\u5316\u5904\u7406\uff0c\u90fd\u5f7b\u5e95\u6539\u53d8\u4e86\u6211\u4eec\u7684\u64cd\u4f5c\u6d41\u7a0b\u3002\u8fd9\u6b21\u66f4\u65b0\u786e\u4fdd\u4e86\u6301\u7eed\u53ef\u9760\u7684\u8f93\u51fa\uff0c\u8ba9\u6211\u4eec\u80fd\u591f\u83b7\u5f97\u66f4\u51fa\u8272\u7684\u7ed3\u679c\uff0c\u589e\u5f3a\u4e86\u6211\u4eec\u5bf9\u6bcf\u6b21\u6d4b\u5e8f\u8fd0\u884c\u7684\u4fe1\u5fc3\u3002”<\/p>\n
\u535a\u5fb7\u7814\u7a76\u6240\u7684Broad Clinical Labs\u4e5f\u53c2\u4e0e\u4e86\u65e9\u671f\u6d4b\u8bd5\u3002\u8be5\u673a\u6784\u7684\u5b9e\u9a8c\u5ba4\u8fd0\u8425\u8d1f\u8d23\u4ebaTom Howd\u79f0\uff1a”\u5728 NovaSeq X Plus \u6d4b\u5e8f\u4eea\u4e0a\u8fdb\u884c\u76841.3 \u7248\u672c\u8f6f\u4ef6\u5347\u7ea7\u65e9\u671f\u6d4b\u8bd5\u4e2d\uff0c\u6211\u4eec\u53d1\u73b0\u4e86\u9ad8\u8d28\u91cf\u6570\u636e\u4ea7\u91cf\u7684\u663e\u8457\u63d0\u5347\u3002\u6211\u4eec\u975e\u5e38\u671f\u5f85\u6240\u6709\u7528\u6237\u90fd\u80fd\u4ece\u8fd9\u4e9b\u4ea7\u80fd\u589e\u76ca\u4e2d\u53d7\u76ca\uff0c\u8fdb\u4e00\u6b65\u63a8\u52a8\u4ed6\u4eec\u7684\u79d1\u5b66\u7814\u7a76\u3002”<\/p>\n
\u4e4c\u7279\u52d2\u652f\u5927\u5b66\u533b\u5b66\u4e2d\u5fc3\u4e34\u5e8a\u9057\u4f20\u5b66\u5b9e\u9a8c\u5ba4\u4e13\u5bb6\u517c NGS \u6838\u5fc3\u8bbe\u65bd\u56e2\u961f\u8d1f\u8d23\u4eba Bert Groet<\/span> \u4e5f\u5bf9\u8fd9\u4e00\u8fdb\u5c55\u8868\u793a\u8ba4\u53ef\uff1a”\u968f\u7740 NovaSeq X Plus \u642d\u8f7d\u8f6f\u4ef6 1.3 \u7248\u672c\u7684\u53d1\u5e03\uff0c\u56e0\u7f8e\u7eb3\u4e3a\u6211\u4eec\u7684\u57fa\u56e0\u7ec4\u5b9e\u9a8c\u5ba4\u5e26\u6765\u4e86\u4ece\u57fa\u4e8e\u5168\u5916\u663e\u5b50\u5206\u6790\u8fc7\u6e21\u5230\u57fa\u4e8e\u5168\u57fa\u56e0\u7ec4\u5206\u6790\u7684\u51b3\u5b9a\u6027\u8fdb\u5c55\u3002\u5728\u4e0d\u5f71\u54cd\u9ad8Q30\u5206\u503c\u7684\u524d\u63d0\u4e0b\uff0c\u6d4b\u5e8f\u603b\u4ea7\u80fd\u5927\u5e45\u63d0\u9ad8\uff0c\u4fdd\u969c\u4e86\u9ad8\u901a\u91cf\u3001\u9ad8\u8d28\u91cf\u7684\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\uff0c\u4ece\u800c\u5e2e\u52a9\u6211\u4eec\u4e3a\u5c06\u6765\u7684\u5ba2\u6237\u63d0\u4f9b\u7b54\u6848\u3002”<\/p>\n25B<\/span> 100\u5faa\u73af\u548c200\u5faa\u73af\u8bd5\u5242\u76d2\u6269\u5c55\u6d4b\u5e8f\u80fd\u529b<\/p>\n\u7528\u4e8eNovaSeq X\u4ea7\u54c1\u7cfb\u5217\u7684\u5168\u65b025B 100\u5faa\u73af\u548c200\u5faa\u73af\u8bd5\u5242\u76d2\u5df2\u4e8e2024\u5e7412 \u6708\u5f00\u59cb\u53d1\u8d27\uff0c\u8fd9\u4e9b\u8bd5\u5242\u76d2\u5bf9\u5927\u89c4\u6a21\u7684\u8ba1\u6570\u5e94\u7528\u53ca\u5176\u4ed6\u68c0\u6d4b\u8fdb\u884c\u4e86\u4f18\u5316\uff0c\u7f29\u77ed\u4e86\u5468\u8f6c\u65f6\u95f4\uff0c\u63d0\u9ad8\u4e86\u8fd0\u884c\u6210\u672c\u6548\u76ca\u3002\u51ed\u501f\u5728\u8fd0\u884c\u89c4\u6a21\u548c\u8bfb\u957f\u65b9\u9762\u7684\u7075\u6d3b\u6027\uff0c25B\u8bd5\u5242\u76d2\u53ef\u6ee1\u8db3\u4e34\u5e8a\u79d1\u7814\u5b9e\u9a8c\u5ba4\u3001\u6838\u5fc3\u5b66\u672f\u5b9e\u9a8c\u5ba4\u548c\u670d\u52a1\u5546\u4e0d\u65ad\u53d1\u5c55\u7684\u9700\u6c42\u3002<\/p>\n
Illumina Single-Cell Prep <\/p>\n
\u4eca\u65e5\uff0c\u56e0\u7f8e\u7eb3\u8fd8\u5ba3\u5e03Illumina Single-Cell Prep\uff08\u524d\u8eab\u4e3aFluent PIPseq V\uff09\u73b0\u5df2\u4e0a\u5e02\uff0c\u5e76\u5c06\u9010\u6b65\u5f00\u59cb\u5411\u5ba2\u6237\u53d1\u8d27\uff0c\u8be5\u4ea7\u54c1\u80fd\u591f\u4e0e\u56e0\u7f8e\u7eb3NextSeq 1000\/2000\u3001NovaSeq 6000 \u548c\u00a0NovaSeq X\u7b49\u65b0\u4e00\u4ee3\u6d4b\u5e8f\u4eea\u5668\u5e7f\u6cdb\u517c\u5bb9\u3002\u8fd9\u9879\u5355\u7ec6\u80de\u6837\u54c1\u5efa\u5e93\u6280\u672f\u7a81\u7834\u4e86\u5f53\u524d\u6d4b\u5e8f\u65b9\u6cd5\u4e2d\u7684\u591a\u91cd\u969c\u788d\u548c\u9650\u5236\uff0c\u5728\u76f8\u540c\u7684\u6d41\u7a0b\u548c\u5468\u8f6c\u65f6\u95f4\u4e0b\uff0c\u5b9e\u73b0\u4e86\u4ece\u6570\u767e\u5230\u6570\u767e\u4e07\u7ec6\u80de\u91cf\u7ea7\u7684\u5b9e\u9a8c\u53ef\u6269\u5c55\u6027\u3002\u4f4e\u8bbe\u5907\u6210\u672c\u3001\u66f4\u4f18\u5355\u4e2a\u7ec6\u80de\u6210\u672c\u4ee5\u53ca\u9ad8\u7075\u654f\u5ea6\u7b49\u7279\u6027\uff0c\u4f7f\u5f97\u521d\u6b21\u63a5\u89e6\u5355\u7ec6\u80de\u6d4b\u5e8f\u6280\u672f\u7684\u5ba2\u6237\u80fd\u591f\u5feb\u901f\u3001\u8f7b\u677e\u5730\u5e94\u7528\u8be5\u6280\u672f\u5e76\u53d6\u5f97\u5353\u8d8a\u8868\u73b0\uff0c\u540c\u65f6\u8d4b\u80fd\u5ba2\u6237\u8fdb\u4e00\u6b65\u6269\u5c55\u5b9e\u9a8c\u89c4\u6a21\uff0c\u901a\u8fc7NovaSeq X 25B\u6d41\u52a8\u69fd\u5c31\u53ef\u5b9e\u73b0\u5177\u6709\u6210\u672c\u6548\u76ca\u7684\u8d85\u5927\u89c4\u6a21\u7ec6\u80de\u5206\u6790\u3002<\/p>\n
\u57282024\u5e7411\u6708\u7684\u7f8e\u56fd\u4eba\u7c7b\u9057\u4f20\u5b66\u4f1a\u5e74\u4f1a\uff08ASHG\uff09\u4e0a\uff0c\u7ebd\u7ea6\u5927\u5b66\u683c\u7f57\u65af\u66fc\u533b\u5b66\u9662\u795e\u7ecf\u79d1\u5b66\u4e0e\u751f\u7406\u5b66\u7cfb\u526f\u6559\u6388Shane Liddelow\u535a\u58eb\u5c55\u793a\u4e86\u5173\u4e8e\u8fd9\u4e00\u521b\u65b0\u6280\u672f\u7684\u65e9\u671f\u6570\u636e\u548c\u6d1e\u5bdf\u3002\u8be5\u6280\u672f\u80fd\u591f\u5bf9\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u3001\u8111\u7ec4\u7ec7\u7ec6\u80de\u7b49\u590d\u6742\u7ec6\u80de\u6216\u6837\u672c\u7c7b\u578b\u8fdb\u884c\u6d4b\u5e8f\uff0c\u6709\u52a9\u4e8e\u89c4\u907f\u57fa\u4e8e\u5fae\u6d41\u63a7\u7684\u5355\u7ec6\u80de\u6280\u672f\u4e2d\u7531\u7ba1\u9053\u5835\u585e\u6216\u5176\u4ed6\u6280\u672f\u969c\u5e26\u6765\u7684\u6837\u672c\u5206\u6790\u96be\u70b9\u3002<\/p>\n
NovaSeq X\u7cfb\u5217\u7684\u5168\u65b025B\u8bd5\u5242\u76d2\u4e0eIllumina Single-Cell Prep\u517c\u5bb9\uff0c\u4e3a\u5927\u89c4\u6a21\u5355\u7ec6\u80de\u5206\u6790\u9879\u76ee\u63d0\u4f9b\u4e86\u9ad8\u6548\u3001\u7ecf\u6d4e\u4e14\u7cbe\u51c6\u7684\u5de5\u4f5c\u6d41\u7a0b\u3002Illumina Single-Cell Prep\u901a\u8fc7\u7b80\u5316\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5de5\u4f5c\u6d41\u7a0b\uff0c\u53ef\u5c06NovaSeq X\u7cfb\u5217\u6d4b\u5e8f\u4eea\u751f\u6210\u7684\u6570\u636e\u76f4\u63a5\u4f20\u8f93\u81f3\u56e0\u7f8e\u7eb3\u4e91\u7aef\u4fe1\u606f\u89e3\u51b3\u65b9\u6848\u4e2d\u7684\u81ea\u52a8\u5316DRAGEN\u5206\u6790\u5e73\u53f0[1]\u3002\u4e0e\u6b64\u540c\u65f6\uff0cPartek Flow\u53ef\u5bf9\u5927\u89c4\u6a21Illumina Single-Cell Prep\u6570\u636e\u96c6\u8fdb\u884c\u5168\u9762\u7684\u53ef\u89c6\u5316\u548c\u89e3\u6790\uff0c\u52a9\u529b\u5dee\u5f02\u5316\u8868\u8fbe\u548c\u751f\u7269\u6807\u5fd7\u7269\u53d1\u73b0\u3002<\/p>\n
\u524d\u77bb\u6027\u58f0\u660e<\/p>\n
\u672c\u65b0\u95fb\u7a3f\u5305\u542b\u6d89\u53ca\u98ce\u9669\u548c\u4e0d\u786e\u5b9a\u6027\u7684\u524d\u77bb\u6027\u58f0\u660e\u3002\u6211\u4eec\u7684\u4e1a\u52a1\u4f1a\u53d7\u5230\u4e00\u4e9b\u91cd\u8981\u56e0\u7d20\u7684\u5f71\u54cd\uff0c\u8fd9\u4e9b\u56e0\u7d20\u53ef\u80fd\u5bfc\u81f4\u5b9e\u9645\u7ed3\u679c\u4e0e\u4efb\u4f55\u524d\u77bb\u6027\u9648\u8ff0\u5b58\u5728\u5b9e\u8d28\u6027\u5dee\u5f02\uff1a\u00a0(i)\u5f00\u53d1\u3001\u5236\u9020\u548c\u63a8\u51fa\u65b0\u4ea7\u54c1\u548c\u670d\u52a1\u6240\u56fa\u6709\u7684\u6311\u6218\uff1b(ii)\u6211\u4eec\u5236\u9020\u575a\u56fa\u8010\u7528\u7684\u4eea\u5668\u548c\u8017\u6750\u7684\u80fd\u529b\uff1b(iii)\u5ba2\u6237\u5bf9\u65b0\u4ea7\u54c1\u548c\u670d\u52a1\u7684\u63a5\u53d7\u7a0b\u5ea6\u548c\u6ee1\u610f\u5ea6\uff1b(iv)\u5ba2\u6237\u91c7\u7528\u65b0\u4ea7\u54c1\u7684\u901f\u5ea6\u548c\u89c4\u6a21\uff0c\u4ee5\u53ca\u6211\u4eec\u5411\u7f8e\u56fd\u8bc1\u5238\u4ea4\u6613\u59d4\u5458\u4f1a\u63d0\u4ea4\u7684\u6587\u4ef6\u4e2d\u8be6\u8ff0\u7684\u5176\u4ed6\u56e0\u7d20\uff0c\u5305\u62ec\u6211\u4eec\u6700\u8fd1\u63d0\u4ea4\u7684\u00a010-K \u8868\u548c\u00a010-Q \u8868\uff0c\u6216\u5728\u516c\u5f00\u7535\u8bdd\u4f1a\u8bae\u4e2d\u62ab\u9732\u7684\u4fe1\u606f\uff0c\u7535\u8bdd\u4f1a\u8bae\u7684\u65e5\u671f\u548c\u65f6\u95f4\u5df2\u4e8b\u5148\u516c\u5e03\u3002\u6211\u4eec\u6ca1\u6709\u4e49\u52a1\uff0c\u4e5f\u4e0d\u6253\u7b97\u66f4\u65b0\u8fd9\u4e9b\u524d\u77bb\u6027\u58f0\u660e\uff0c\u5ba1\u67e5\u6216\u786e\u8ba4\u5206\u6790\u5e08\u7684\u9884\u671f\uff0c\u6216\u63d0\u4f9b\u6709\u5173\u672c\u5b63\u5ea6\u8fdb\u5c55\u7684\u4e2d\u671f\u62a5\u544a\u6216\u66f4\u65b0\u3002<\/p>\n
\u5173\u4e8e\u56e0\u7f8e\u7eb3<\/p>\n
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