<\/a><\/p>\n<\/div>\n\u7f8e\u56fd\u4e1c\u90e8\u65f6\u95f42\u670824\u65e5\u661f\u671f\u4e00\u4e0b\u53484:30\u81f34:50\uff0cKingsmore\u535a\u58eb\u5c06\u5728AGBT\u5e76\u884c\u4f1a\u8bae\u4e0a\u53d1\u8868\u9898\u4e3a\u300a\u65b0\u578b\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u5de5\u4f5c\u6d41\u7a0b\u52a9\u529b\u8bbe\u8ba1\u9488\u5bf9\u7f55\u89c1\u9057\u4f20\u75c5\u60a3\u8005\u7684\u7b49\u4f4d\u57fa\u56e0\u7279\u5f02\u6027\u53cd\u4e49\u5be1\u6838\u82f7\u9178\u300b\u7684\u6458\u8981\u3002\u57fa\u4e8e”\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6”\u6280\u672f\u7684\u4ea7\u54c1\u9884\u8ba1\u5c06\u4e8e2026\u5e74\u8fdb\u884c\u5546\u4e1a\u53d1\u5e03\u3002<\/p>\n
\u56e0\u7f8e\u7eb3\u63a8\u51fa\u9762\u5411CRISPR\u7814\u7a76\u7684\u5355\u7ec6\u80de\u89e3\u51b3\u65b9\u6848\u3002\u5355\u7ec6\u80de\u6280\u672f\u6709\u671b\u4e3a\u80bf\u7624\u5b66\u3001\u514d\u75ab\u5b66\u548c\u836f\u7269\u9776\u70b9\u53d1\u73b0\u7b49\u5e7f\u6cdb\u7684\u7814\u7a76\u9886\u57df\u5e26\u6765\u91cd\u8981\u7684\u751f\u7269\u5b66\u89c1\u89e3\u3002\u5168\u65b0\u00a0Perturb-seq \u89e3\u51b3\u65b9\u6848\u4ee5\u524d\u6240\u672a\u6709\u7684\u89c4\u6a21\u4e3a\u56e0\u7f8e\u7eb3\u7684\u591a\u7ec4\u5b66\u4ea7\u54c1\u7ec4\u5408\u5e26\u6765\u4e86\u00a0Perturb-seq \u5b9e\u9a8c\u7684\u65b0\u529f\u80fd\uff0c\u4ece\u800c\u4f7f\u5f97\u5728\u5355\u7ec6\u80de\u5c42\u9762\u4e0a\u53d1\u73b0\u836f\u7269\u4f5c\u7528\u673a\u5236\uff0c\u5e76\u5bf9\u590d\u6742\u9057\u4f20\u75be\u75c5\u7684\u6cbb\u7597\u65b9\u6cd5\u8fdb\u884c\u4e34\u5e8a\u7814\u7a76\u6210\u4e3a\u53ef\u80fd\u3002\u8fd9\u9879\u6280\u672f\u8fd8\u5c06\u7528\u4e8e\u4e0a\u5468\u5ba3\u5e03\u7684\u4e0e\u535a\u5fb7\u4e34\u5e8a\u5b9e\u9a8c\u5ba4\uff08Broad Clinical Labs\uff09\u00a0\u7684\u5408\u4f5c\uff0c\u65e8\u5728\u5feb\u901f\u6269\u5c55\u5355\u7ec6\u80de\u89e3\u51b3\u65b9\u6848\uff0c\u5e76\u5728\u4e09\u5e74\u5185\u5b9e\u73b0\u00a050 \u4ebf\u5355\u7ec6\u80de\u56fe\u8c31\u751f\u6001\u7cfb\u7edf\u3002<\/p>\n
\u8be5\u89e3\u51b3\u65b9\u6848\u9884\u8ba1\u5c06\u57282025\u5e74\u5e95\u63a8\u51fa\uff0c\u63d0\u4f9b\u4e09\u79cd\u65b0\u7684\u8bd5\u5242\u76d2\u578b\u53f7\uff0c\u5206\u522b\u53ef\u517c\u5bb9\u6bcf\u4efd\u6837\u672c10,000\u3001100,000\u548c1,000,000\u4e2a\u7ec6\u80de\uff0c\u4ee5\u652f\u6301\u4ece100\u4e2a\u5f15\u5bfcRNA\uff08gRNA\uff09\u5e8f\u5217\u7684\u9776\u5411\u7814\u7a76\u5230\u5355\u6b21\u5b9e\u9a8c\u8d85\u8fc710,000\u4e2agRNA\u7684\u5168\u57fa\u56e0\u7ec4\u89c4\u6a21\u7684\u53d1\u73b0\u3002\u8be5\u89e3\u51b3\u65b9\u6848\u5229\u7528\u5bf9\u00a0Fluent PIPseq \u57fa\u7840\u6280\u672f\u7684\u6700\u65b0\u6539\u8fdb\uff0c\u5728\u540c\u4e00\u68c0\u6d4b\u4e2d\u5bf9\u00a0CRISPR gRNA \u5e8f\u5217\u548c\u00a03′ RNA \u57fa\u56e0\u8868\u8fbe\u8fdb\u884c\u53cc\u91cd\u6d4b\u91cf\u3002\u4e0e\u76ee\u524d\u7684\u5355\u7ec6\u80de\u8bd5\u5242\u76d2\u76f8\u6bd4\uff0cgRNA \u76f4\u63a5\u6355\u83b7\u5e26\u6765\u4e86\u66f4\u5177\u6210\u672c\u6548\u76ca\u7684\u5de5\u4f5c\u6d41\u7a0b\u3001\u66f4\u9ad8\u7684\u7075\u654f\u5ea6\u548c\u66f4\u5f3a\u5927\u7684\u53ef\u6269\u5c55\u6027\u3002<\/p>\n
\u8be5\u89e3\u51b3\u65b9\u6848\u5c06\u63d0\u4f9bCRISPR\u6fc0\u6d3b\u3001\u5931\u6d3b\u6216\u9776\u5411\u57fa\u56e0\u7f16\u8f91Perturb-seq\u5b9e\u9a8c\u7684\u5355\u7ec6\u80de\u8bfb\u6570\uff0c\u7528\u4e8e\u7ec6\u80de\u56fe\u8c31\u7ed8\u5236\u6216\u836f\u7269\u7b5b\u9009\u7814\u7a76\u7b49\u632f\u594b\u4eba\u5fc3\u7684\u5e94\u7528\u3002\u8fd9\u4e9b\u8bd5\u5242\u76d2\u517c\u5bb9\u6240\u6709\u56e0\u7f8e\u7eb3\u6d4b\u5e8f\u4eea\uff0c\u5e76\u4e14\u5de5\u4f5c\u6d41\u7a0b\u5305\u62ec\u901a\u8fc7\u56e0\u7f8e\u7eb3\u8f6f\u4ef6\u8fdb\u884c\u00a0CRISPR gRNA \u7b5b\u9009\u7684\u00a0DRAGEN \u5355\u7ec6\u80de\u00a0RNA \u5206\u6790\u3002<\/p>\n
\u56e0\u7f8e\u7eb3\u76845\u78b1\u57fa\u89e3\u51b3\u65b9\u6848\u7528\u4e8e\u7532\u57fa\u5316\u5206\u6790\u548c\u53d8\u5f02\u68c0\u6d4b\uff0c\u63d0\u5347\u4e86\u9057\u4f20\u53ca\u8868\u89c2\u9057\u4f20\u6570\u636e\u7684\u51c6\u786e\u6027\u3002\u56e0\u7f8e\u7eb3\u7684\u00a05 \u78b1\u57fa\u7532\u57fa\u5316\u5206\u6790\u89e3\u51b3\u65b9\u6848\u76ee\u524d\u5904\u4e8e\u65e9\u671f\u8bd5\u7528\u9636\u6bb5\uff0c\u53ef\u5728\u4e00\u6b21\u68c0\u6d4b\u4e2d\u540c\u65f6\u8fdb\u884c\u57fa\u56e0\u53d8\u5f02\u548c\u7532\u57fa\u5316\u68c0\u6d4b\u3002DNA \u7532\u57fa\u5316\u4f1a\u5f71\u54cd\u57fa\u56e0\u7684\u8868\u8fbe\u65b9\u5f0f\u548c\u65f6\u95f4\u3002\u68c0\u6d4b\u548c\u5206\u6790\u00a0DNA \u7532\u57fa\u5316\u7684\u80fd\u529b\u80fd\u591f\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u6df1\u5316\u5bf9\u00a0DNA \u7532\u57fa\u5316\u6a21\u5f0f\u5982\u4f55\u5f71\u54cd\u53d1\u80b2\u3001\u7ec6\u80de\u5206\u5316\u3001\u80bf\u7624\u8fdb\u5c55\u548c\u73af\u5883\u66b4\u9732\u7b49\u751f\u7269\u5b66\u8fc7\u7a0b\u7684\u7406\u89e3\u3002\u6700\u7ec8\uff0c\u5b83\u53ef\u4ee5\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u786e\u5b9a\u7528\u4e8e\u68c0\u6d4b\u3001\u8bca\u65ad\u548c\u6cbb\u7597\u7b56\u7565\u7684\u6f5c\u5728\u751f\u7269\u6807\u5fd7\u7269\u3002<\/p>\n
\u56e0\u7f8e\u7eb3\u7684\u89e3\u51b3\u65b9\u6848\u9884\u8ba1\u4e8e2026\u5e74\u63a8\u51fa\uff0c\u5b83\u5c06\u901a\u8fc7\u5355\u6b21\u6587\u5e93\u5236\u5907\u3001\u6d4b\u5e8f\u548c\u5206\u6790\u8fd0\u884c\uff0c\u63d0\u4f9b\u5bf9\u57fa\u56e0\u7ec4\u3001\u65e0\u7ec6\u80deDNA\u6216\u9776\u5411\u6d4b\u5b9a\u76845\u78b1\u57fa\u6d1e\u5bdf\uff0c\u4ece\u800c\u5728\u4fdd\u6301\u6210\u672c\u6548\u76ca\u7684\u540c\u65f6\u52a0\u6df1\u5bf9\u57fa\u56e0\u7ec4\u7684\u7406\u89e3\u3002\u76ee\u524d\uff0c\u91c7\u7528\u00a05 \u78b1\u57fa\u89e3\u51b3\u65b9\u6848\u7684\u884c\u4e1a\u969c\u788d\u5305\u62ec\u6210\u672c\u3001\u53ef\u7528\u6027\u548c\u7f3a\u4e4f\u53ef\u9760\u7684\u5206\u6790\u89e3\u51b3\u65b9\u6848\u3002\u73b0\u6709\u7684\u00a0DNA \u548c\u7532\u57fa\u5316\u540c\u6b65\u68c0\u6d4b\u4ea7\u54c1\u5728\u9057\u4f20\u548c\u8868\u89c2\u9057\u4f20\u6570\u636e\u7684\u51c6\u786e\u6027\u65b9\u9762\u5b58\u5728\u5c40\u9650\u6027\u3002\u56e0\u7f8e\u7eb3\u7684\u89e3\u51b3\u65b9\u6848\u65e8\u5728\u901a\u8fc7\u63d0\u4f9b\u66f4\u52a0\u7b80\u4fbf\u3001\u66f4\u5177\u6210\u672c\u6548\u76ca\u7684\u7aef\u5230\u7aef\u89e3\u51b3\u65b9\u6848\u6765\u89e3\u51b3\u8fd9\u4e9b\u969c\u788d\uff0c\u4ece\u800c\u5728\u5355\u6b21\u5b9e\u9a8c\u4e2d\u5b9e\u73b0\u9057\u4f20\u53d8\u5f02\u548c\u7532\u57fa\u5316\u7684\u9ad8\u7cbe\u5ea6\u68c0\u6d4b\u3002<\/p>\n
\u7f8e\u56fd\u4e1c\u90e8\u65f6\u95f42\u670825\u65e5\u661f\u671f\u4e8c\u665a\u4e0a9:10\u81f39:30\u7684AGBT\u5e76\u884c\u4f1a\u8bae\u4e0a\uff0c\u4f26\u6566\u5065\u5eb7\u79d1\u5b66\u4e2d\u5fc3\u7814\u7a76\u6240\u5206\u5b50\u9057\u4f20\u5b66\u5b9e\u9a8c\u5ba4\u4e3b\u4efbBekim Sadikovic\u535a\u58eb\u5c06\u4ee5\u300a\u7528\u4e8e\u7f55\u89c1\u672a\u786e\u8bca\u9057\u4f20\u75c5\u7684\u9057\u4f20\u53d8\u5f02\u548c\u8868\u89c2\u9057\u4f20\u6807\u5fd7\u7269\u540c\u65f6\u68c0\u6d4b\u7684\u65b0\u96c6\u6210\u5e73\u53f0\u300b\u4e3a\u9898\uff0c\u53d1\u8868\u8be5\u89e3\u51b3\u65b9\u6848\u7684\u65e9\u671f\u8bd5\u7528\u5ba2\u6237\u6570\u636e\u3002<\/p>\n
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2025\u5e742\u670824\u65e5\uff0c\u56e0\u7f8e\u7eb3\u516c\u53f8\uff08\u7eb3\u65af\u8fbe\u514b\u80a1\u7968\u4ee3\u7801\uff1aILMN\uff09\u63a8\u51fa\u4e00\u7cfb\u5217\u6280\u672f\u8def\u7ebf\u56fe\u521b\u65b0\uff0c\u5efa\u7acb\u4e86\u4e1a\u5185\u6700\u5927\u7684\u7ec4\u5b66\u89e3\u51b3\u65b9\u6848\u548c\u6d4b\u5e8f\u5e94\u7528\u4ea7\u54c1\u7ec4\u5408\u3002\u8fd9\u4e9b\u89e3\u51b3\u65b9\u6848\u6db5\u76d6\u4e86\u57fa\u56e0\u7ec4\u5b66\u3001\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u3001\u5355\u7ec6\u80de\u5206\u6790\u3001CRISPR\u6280\u672f\u3001\u8868\u89c2\u9057\u4f20\u5b66\u548c\u6570\u636e\u5206\u6790\u8f6f\u4ef6\uff0c\u5c06\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u6df1\u5165\u4e86\u89e3\u75be\u75c5\u7684\u9a71\u52a8\u56e0\u7d20\u3002\u57fa\u4e8e\u62e5\u6709\u5353\u8d8a\u8d28\u91cf\u3001\u4e00\u81f4\u6027\u548c\u51c6\u786e\u6027\u7684\u56e0\u7f8e\u7eb3\u6d4b\u5e8f\u4eea\uff0c\u8fd9\u4e9b\u65b0\u578b\u7ec4\u5b66\u89e3\u51b3\u65b9\u6848\u5728\u89c4\u6a21\u3001\u51c6\u786e\u6027\u548c\u53ef\u9760\u6027\u65b9\u9762\u5747\u8d85\u8d8a\u4e86\u884c\u4e1a\u6807\u51c6\u3002\u6269\u5c55\u540e\u7684\u591a\u7ec4\u5b66\u4ea7\u54c1\u7ec4\u5408\u5c06\u4e8e\u7f8e\u56fd\u4e1c\u90e8\u65f6\u95f42\u670824\u65e5\u5728\u57fa\u56e0\u7ec4\u751f\u7269\u5b66\u6280\u672f\u8fdb\u5c55\u5927\u4f1a\uff08AGBT\uff09\u4e0a\u53d1\u5e03\u3002 \u56e0\u7f8e\u7eb3\u9996\u5e2d\u6267\u884c\u5b98Jacob Thaysen\u8868\u793a\uff0c”\u56e0\u7f8e\u7eb3\u662f\u884c\u4e1a\u521b\u65b0\u7684\u4e2d\u575a\u529b\u91cf\uff0c\u6211\u4eec\u5c06\u51dd\u805a\u56e0\u7f8e\u7eb3\u72ec\u6709\u7684\u89c4\u6a21\u548c\u5f71\u54cd\u529b\uff0c\u5e26\u6765\u7a81\u7834\u6027\u7684\u5168\u65b0\u89e3\u51b3\u65b9\u6848\u3002\u56e0\u7f8e\u7eb3\u59cb\u7ec8\u81f4\u529b\u4e8e\u4e3a\u57fa\u56e0\u7ec4\u5b66\u7a81\u7834\u5f00\u8f9f\u65b0\u8def\u5f84\uff0c\u73b0\u5728\u6211\u4eec\u81f4\u529b\u4e8e\u52a0\u901f\u591a\u7ec4\u5b66\u5404\u4e2a\u9886\u57df\u7684\u8fdb\u5c55\u3002\u6211\u4eec\u7684\u6280\u672f\u5c06\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u66f4\u5168\u9762\u5730\u4e86\u89e3\u751f\u7269\u5b66\uff0c\u52a0\u5feb\u5c06\u6570\u636e\u8f6c\u5316\u4e3a\u6d1e\u5bdf\uff0c\u4ece\u800c\u771f\u6b63\u5b9e\u73b0\u7cbe\u51c6\u533b\u7597\u3002” \u56e0\u7f8e\u7eb3\u9996\u5e2d\u6280\u672f\u5b98Steve Barnard\u535a\u58eb\u8868\u793a\uff0c”\u56e0\u7f8e\u7eb3\u4e00\u5982\u65e2\u5f80\u5730\u63a8\u52a8\u5f00\u62d3\u6027\u521b\u65b0\u5e76\u5851\u9020\u884c\u4e1a\u6807\u6746\u3002\u6b63\u5982\u7814\u7a76\u4eba\u5458\u6240\u4fe1\u8d56\u7684\u51c6\u786e\u3001\u5b8c\u6574\u4e14\u5168\u9762\u7684\u57fa\u56e0\u7ec4\u89c6\u56fe\u4e00\u6837\uff0c\u56e0\u7f8e\u7eb3\u73b0\u5728\u5c06\u8fd9\u4e9b\u5c5e\u6027\u5f15\u5165\u6574\u4e2a\u591a\u7ec4\u5b66\u9886\u57df\u7684\u63a2\u7d22\u4e2d\uff0c\u5e76\u63d0\u4f9b\u4e86\u5145\u5206\u4fdd\u969c\u4ef7\u503c\u4e0e\u53ef\u7528\u6027\u7684\u5b8c\u6574\u5de5\u4f5c\u6d41\u7a0b\u3002\u8fd9\u4e9b\u89e3\u51b3\u65b9\u6848\u662f\u79d1\u7814\u53d1\u73b0\u7684\u4e00\u6b21\u98de\u8dc3\uff0c\u6211\u4eec\u975e\u5e38\u671f\u5f85\u5c06\u5176\u5e26\u5165\u66f4\u591a\u7684\u5b9e\u9a8c\u5ba4\u3002” \u56e0\u7f8e\u7eb3\u5728AGBT\u4e0a\u7684\u7cfb\u5217\u53d1\u5e03\u9a71\u52a8\u591a\u7ec4\u5b66\u53d1\u73b0\u89c4\u6a21\u4e0e\u8d28\u91cf\u63d0\u5347 \u5728AGBT\u7814\u8ba8\u4f1a\u4e0a\uff0c\u56e0\u7f8e\u7eb3\u5c06\u5c55\u793a\u57fa\u4e8e\u5176\u6d4b\u5e8f\u5e73\u53f0\u53ca\u5168\u65b0\u591a\u6a21\u6001\u6570\u636e\u5206\u6790\u5e73\u53f0\u7684\u591a\u7ec4\u5b66\u80fd\u529b\u7684\u6700\u65b0\u8fdb\u5c55\u3002\u8fd9\u4e9b\u8fdb\u5c55\u8868\u660e\uff0c\u56e0\u7f8e\u7eb3\u6301\u7eed\u81f4\u529b\u4e8e\u4e3a\u5ba2\u6237\u63d0\u4f9b\u7b80\u5316\u5de5\u4f5c\u6d41\u7a0b\u7684\u7aef\u5230\u7aef\u89e3\u51b3\u65b9\u6848\uff0c\u5e76\u4e0d\u65ad\u6269\u5927\u5176\u591a\u7ec4\u5b66\u4ea7\u54c1\u7ec4\u5408\uff0c\u5176\u4e2d\u5305\u62ec\u00a0Illumina Single Cell \u548c\u00a0Illumina Protein Prep\u3002 “\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6\u6280\u672f”\u89e3\u9501\u7f55\u89c1\u75c5\u68c0\u6d4b\u7684\u5168\u65b0\u53ef\u80fd\u3002\u56e0\u7f8e\u7eb3\u7684”\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6\uff08constellation mapped read\uff09”\u6280\u672f\u65e8\u5728\u52a0\u6df1\u5bf9\u57fa\u56e0\u7ec4\u7684\u7406\u89e3\uff0c\u5e76\u7b80\u5316\u5168\u57fa\u56e0\u7ec4\u5de5\u4f5c\u6d41\u7a0b\uff0c\u540c\u65f6\u51cf\u5c11\u4f20\u7edf\u7684\u6587\u5e93\u5236\u5907\u3002\u8be5\u6280\u672f\u76ee\u524d\u6b63\u5728\u5f00\u53d1\u4e2d\uff0c\u65e9\u671f\u6d4b\u8bd5\u8868\u660e\uff0c\u5176\u5728\u590d\u6742\u57fa\u56e0\u7ec4\u533a\u57df\u3001\u8d85\u957f\u76f8\u4f4d\u548c\u5927\u578b\u7ed3\u6784\u91cd\u6392\u65b9\u9762\u7684\u68c0\u6d4b\u80fd\u529b\u5f97\u5230\u4e86\u589e\u5f3a\u3002\u8fd9\u4e9b\u8fdb\u6b65\u6709\u671b\u4e3a\u66f4\u52a0\u5feb\u901f\u3001\u5168\u9762\u7684\u75be\u75c5\u68c0\u6d4b\u63d0\u4f9b\u652f\u6301\u3002 Rady\u513f\u7ae5\u57fa\u56e0\u7ec4\u533b\u5b66\u7814\u7a76\u6240\u603b\u88c1\u517c\u9996\u5e2d\u6267\u884c\u5b98Stephen Kingsmore\u535a\u58eb\u5c06\u5206\u4eab\u5176\u5bf9”\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6”\u6280\u672f\u7684\u7ecf\u9a8c\uff0c\u4ee5\u53ca\u8be5\u6280\u672f\u5bf9\u5168\u9762\u3001\u5feb\u901f\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u7684\u6f5c\u5728\u5f71\u54cd\uff0c\u4ece\u800c\u5b9e\u73b0\u66f4\u65e9\u7684\u9057\u4f20\u548c\u7f55\u89c1\u75be\u75c5\u8bca\u65ad\u3002 “\u2018\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6’\u6280\u672f\u80fd\u591f\u63d0\u4f9b\u7ed3\u6784\u6027\u53d8\u5f02\u7684\u9ad8\u5206\u8fa8\u7387\u89c6\u56fe\u2014\u2014\u5305\u62ec\u5bfc\u81f4\u7537\u5b69A\u578b\u8840\u53cb\u75c5\u7684\u6700\u5e38\u89c1\u539f\u56e0\uff0c\u8fd9\u662f\u6807\u51c6\u65b9\u6cd5\u76ee\u524d\u65e0\u6cd5\u5b9e\u73b0\u7684\u2014\u2014\u8fd9\u662f\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u9886\u57df\u4ee4\u4eba\u5370\u8c61\u6df1\u523b\u7684\u8fdb\u6b65\uff0c”\u00a0Kingsmore\u8868\u793a\uff0c”\u4ee5\u524d\uff0c\u8fd9\u4e9b\u53d8\u5f02\u5fc5\u987b\u901a\u8fc7\u5b9a\u5236\u7684\u4e00\u6b21\u6027\u6d4b\u5b9a\u6765\u786e\u5b9a\uff0c\u901a\u5e38\u9700\u8981\u4e00\u4e2a\u6708\u6216\u66f4\u957f\u65f6\u95f4\u3002\u2018\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6’\u6280\u672f\u8ba9\u6211\u4eec\u80fd\u591f\u5728\u4ec5\u4e00\u5929\u591a\u7684\u65f6\u95f4\u5185\u5b8c\u6210\u4ece\u5feb\u901f\u6d4b\u5e8f\u5230\u6df1\u5165\u5206\u6790\u7684\u8fc7\u7a0b\u3002” \u7f8e\u56fd\u4e1c\u90e8\u65f6\u95f42\u670824\u65e5\u661f\u671f\u4e00\u4e0b\u53484:30\u81f34:50\uff0cKingsmore\u535a\u58eb\u5c06\u5728AGBT\u5e76\u884c\u4f1a\u8bae\u4e0a\u53d1\u8868\u9898\u4e3a\u300a\u65b0\u578b\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u5de5\u4f5c\u6d41\u7a0b\u52a9\u529b\u8bbe\u8ba1\u9488\u5bf9\u7f55\u89c1\u9057\u4f20\u75c5\u60a3\u8005\u7684\u7b49\u4f4d\u57fa\u56e0\u7279\u5f02\u6027\u53cd\u4e49\u5be1\u6838\u82f7\u9178\u300b\u7684\u6458\u8981\u3002\u57fa\u4e8e”\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6”\u6280\u672f\u7684\u4ea7\u54c1\u9884\u8ba1\u5c06\u4e8e2026\u5e74\u8fdb\u884c\u5546\u4e1a\u53d1\u5e03\u3002 \u56e0\u7f8e\u7eb3\u63a8\u51fa\u9762\u5411CRISPR\u7814\u7a76\u7684\u5355\u7ec6\u80de\u89e3\u51b3\u65b9\u6848\u3002\u5355\u7ec6\u80de\u6280\u672f\u6709\u671b\u4e3a\u80bf\u7624\u5b66\u3001\u514d\u75ab\u5b66\u548c\u836f\u7269\u9776\u70b9\u53d1\u73b0\u7b49\u5e7f\u6cdb\u7684\u7814\u7a76\u9886\u57df\u5e26\u6765\u91cd\u8981\u7684\u751f\u7269\u5b66\u89c1\u89e3\u3002\u5168\u65b0\u00a0Perturb-seq \u89e3\u51b3\u65b9\u6848\u4ee5\u524d\u6240\u672a\u6709\u7684\u89c4\u6a21\u4e3a\u56e0\u7f8e\u7eb3\u7684\u591a\u7ec4\u5b66\u4ea7\u54c1\u7ec4\u5408\u5e26\u6765\u4e86\u00a0Perturb-seq \u5b9e\u9a8c\u7684\u65b0\u529f\u80fd\uff0c\u4ece\u800c\u4f7f\u5f97\u5728\u5355\u7ec6\u80de\u5c42\u9762\u4e0a\u53d1\u73b0\u836f\u7269\u4f5c\u7528\u673a\u5236\uff0c\u5e76\u5bf9\u590d\u6742\u9057\u4f20\u75be\u75c5\u7684\u6cbb\u7597\u65b9\u6cd5\u8fdb\u884c\u4e34\u5e8a\u7814\u7a76\u6210\u4e3a\u53ef\u80fd\u3002\u8fd9\u9879\u6280\u672f\u8fd8\u5c06\u7528\u4e8e\u4e0a\u5468\u5ba3\u5e03\u7684\u4e0e\u535a\u5fb7\u4e34\u5e8a\u5b9e\u9a8c\u5ba4\uff08Broad Clinical Labs\uff09\u00a0\u7684\u5408\u4f5c\uff0c\u65e8\u5728\u5feb\u901f\u6269\u5c55\u5355\u7ec6\u80de\u89e3\u51b3\u65b9\u6848\uff0c\u5e76\u5728\u4e09\u5e74\u5185\u5b9e\u73b0\u00a050 \u4ebf\u5355\u7ec6\u80de\u56fe\u8c31\u751f\u6001\u7cfb\u7edf\u3002 \u8be5\u89e3\u51b3\u65b9\u6848\u9884\u8ba1\u5c06\u57282025\u5e74\u5e95\u63a8\u51fa\uff0c\u63d0\u4f9b\u4e09\u79cd\u65b0\u7684\u8bd5\u5242\u76d2\u578b\u53f7\uff0c\u5206\u522b\u53ef\u517c\u5bb9\u6bcf\u4efd\u6837\u672c10,000\u3001100,000\u548c1,000,000\u4e2a\u7ec6\u80de\uff0c\u4ee5\u652f\u6301\u4ece100\u4e2a\u5f15\u5bfcRNA\uff08gRNA\uff09\u5e8f\u5217\u7684\u9776\u5411\u7814\u7a76\u5230\u5355\u6b21\u5b9e\u9a8c\u8d85\u8fc710,000\u4e2agRNA\u7684\u5168\u57fa\u56e0\u7ec4\u89c4\u6a21\u7684\u53d1\u73b0\u3002\u8be5\u89e3\u51b3\u65b9\u6848\u5229\u7528\u5bf9\u00a0Fluent PIPseq \u57fa\u7840\u6280\u672f\u7684\u6700\u65b0\u6539\u8fdb\uff0c\u5728\u540c\u4e00\u68c0\u6d4b\u4e2d\u5bf9\u00a0CRISPR gRNA \u5e8f\u5217\u548c\u00a03′ RNA \u57fa\u56e0\u8868\u8fbe\u8fdb\u884c\u53cc\u91cd\u6d4b\u91cf\u3002\u4e0e\u76ee\u524d\u7684\u5355\u7ec6\u80de\u8bd5\u5242\u76d2\u76f8\u6bd4\uff0cgRNA \u76f4\u63a5\u6355\u83b7\u5e26\u6765\u4e86\u66f4\u5177\u6210\u672c\u6548\u76ca\u7684\u5de5\u4f5c\u6d41\u7a0b\u3001\u66f4\u9ad8\u7684\u7075\u654f\u5ea6\u548c\u66f4\u5f3a\u5927\u7684\u53ef\u6269\u5c55\u6027\u3002 \u8be5\u89e3\u51b3\u65b9\u6848\u5c06\u63d0\u4f9bCRISPR\u6fc0\u6d3b\u3001\u5931\u6d3b\u6216\u9776\u5411\u57fa\u56e0\u7f16\u8f91Perturb-seq\u5b9e\u9a8c\u7684\u5355\u7ec6\u80de\u8bfb\u6570\uff0c\u7528\u4e8e\u7ec6\u80de\u56fe\u8c31\u7ed8\u5236\u6216\u836f\u7269\u7b5b\u9009\u7814\u7a76\u7b49\u632f\u594b\u4eba\u5fc3\u7684\u5e94\u7528\u3002\u8fd9\u4e9b\u8bd5\u5242\u76d2\u517c\u5bb9\u6240\u6709\u56e0\u7f8e\u7eb3\u6d4b\u5e8f\u4eea\uff0c\u5e76\u4e14\u5de5\u4f5c\u6d41\u7a0b\u5305\u62ec\u901a\u8fc7\u56e0\u7f8e\u7eb3\u8f6f\u4ef6\u8fdb\u884c\u00a0CRISPR gRNA \u7b5b\u9009\u7684\u00a0DRAGEN \u5355\u7ec6\u80de\u00a0RNA \u5206\u6790\u3002 \u56e0\u7f8e\u7eb3\u76845\u78b1\u57fa\u89e3\u51b3\u65b9\u6848\u7528\u4e8e\u7532\u57fa\u5316\u5206\u6790\u548c\u53d8\u5f02\u68c0\u6d4b\uff0c\u63d0\u5347\u4e86\u9057\u4f20\u53ca\u8868\u89c2\u9057\u4f20\u6570\u636e\u7684\u51c6\u786e\u6027\u3002\u56e0\u7f8e\u7eb3\u7684\u00a05 \u78b1\u57fa\u7532\u57fa\u5316\u5206\u6790\u89e3\u51b3\u65b9\u6848\u76ee\u524d\u5904\u4e8e\u65e9\u671f\u8bd5\u7528\u9636\u6bb5\uff0c\u53ef\u5728\u4e00\u6b21\u68c0\u6d4b\u4e2d\u540c\u65f6\u8fdb\u884c\u57fa\u56e0\u53d8\u5f02\u548c\u7532\u57fa\u5316\u68c0\u6d4b\u3002DNA \u7532\u57fa\u5316\u4f1a\u5f71\u54cd\u57fa\u56e0\u7684\u8868\u8fbe\u65b9\u5f0f\u548c\u65f6\u95f4\u3002\u68c0\u6d4b\u548c\u5206\u6790\u00a0DNA \u7532\u57fa\u5316\u7684\u80fd\u529b\u80fd\u591f\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u6df1\u5316\u5bf9\u00a0DNA 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Illumina Connected […]<\/p>\n","protected":false},"author":0,"featured_media":7720,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[25],"tags":[],"class_list":["post-7719","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-pr-newswire"],"yoast_head":"\n
\u56e0\u7f8e\u7eb3\u5168\u65b0\u6280\u672f\u63a8\u52a8\u591a\u7ec4\u5b66\u7814\u7a76\u9769\u65b0\uff0c\u52a9\u529b\u89e3\u9501\u66f4\u6df1\u5165\u7684\u751f\u7269\u5b66\u89c1\u89e3 - Money Compass CN<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/moneycompass.com.my\/cn\/\u56e0\u7f8e\u7eb3\u5168\u65b0\u6280\u672f\u63a8\u52a8\u591a\u7ec4\u5b66\u7814\u7a76\u9769\u65b0\uff0c\u52a9\u529b\u89e3\u9501\u66f4\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u56e0\u7f8e\u7eb3\u5168\u65b0\u6280\u672f\u63a8\u52a8\u591a\u7ec4\u5b66\u7814\u7a76\u9769\u65b0\uff0c\u52a9\u529b\u89e3\u9501\u66f4\u6df1\u5165\u7684\u751f\u7269\u5b66\u89c1\u89e3 - Money Compass CN\" \/>\n<meta property=\"og:description\" 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2025\u5e742\u670824\u65e5\uff0c\u56e0\u7f8e\u7eb3\u516c\u53f8\uff08\u7eb3\u65af\u8fbe\u514b\u80a1\u7968\u4ee3\u7801\uff1aILMN\uff09\u63a8\u51fa\u4e00\u7cfb\u5217\u6280\u672f\u8def\u7ebf\u56fe\u521b\u65b0\uff0c\u5efa\u7acb\u4e86\u4e1a\u5185\u6700\u5927\u7684\u7ec4\u5b66\u89e3\u51b3\u65b9\u6848\u548c\u6d4b\u5e8f\u5e94\u7528\u4ea7\u54c1\u7ec4\u5408\u3002\u8fd9\u4e9b\u89e3\u51b3\u65b9\u6848\u6db5\u76d6\u4e86\u57fa\u56e0\u7ec4\u5b66\u3001\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u3001\u5355\u7ec6\u80de\u5206\u6790\u3001CRISPR\u6280\u672f\u3001\u8868\u89c2\u9057\u4f20\u5b66\u548c\u6570\u636e\u5206\u6790\u8f6f\u4ef6\uff0c\u5c06\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u6df1\u5165\u4e86\u89e3\u75be\u75c5\u7684\u9a71\u52a8\u56e0\u7d20\u3002\u57fa\u4e8e\u62e5\u6709\u5353\u8d8a\u8d28\u91cf\u3001\u4e00\u81f4\u6027\u548c\u51c6\u786e\u6027\u7684\u56e0\u7f8e\u7eb3\u6d4b\u5e8f\u4eea\uff0c\u8fd9\u4e9b\u65b0\u578b\u7ec4\u5b66\u89e3\u51b3\u65b9\u6848\u5728\u89c4\u6a21\u3001\u51c6\u786e\u6027\u548c\u53ef\u9760\u6027\u65b9\u9762\u5747\u8d85\u8d8a\u4e86\u884c\u4e1a\u6807\u51c6\u3002\u6269\u5c55\u540e\u7684\u591a\u7ec4\u5b66\u4ea7\u54c1\u7ec4\u5408\u5c06\u4e8e\u7f8e\u56fd\u4e1c\u90e8\u65f6\u95f42\u670824\u65e5\u5728\u57fa\u56e0\u7ec4\u751f\u7269\u5b66\u6280\u672f\u8fdb\u5c55\u5927\u4f1a\uff08AGBT\uff09\u4e0a\u53d1\u5e03\u3002 \u56e0\u7f8e\u7eb3\u9996\u5e2d\u6267\u884c\u5b98Jacob Thaysen\u8868\u793a\uff0c”\u56e0\u7f8e\u7eb3\u662f\u884c\u4e1a\u521b\u65b0\u7684\u4e2d\u575a\u529b\u91cf\uff0c\u6211\u4eec\u5c06\u51dd\u805a\u56e0\u7f8e\u7eb3\u72ec\u6709\u7684\u89c4\u6a21\u548c\u5f71\u54cd\u529b\uff0c\u5e26\u6765\u7a81\u7834\u6027\u7684\u5168\u65b0\u89e3\u51b3\u65b9\u6848\u3002\u56e0\u7f8e\u7eb3\u59cb\u7ec8\u81f4\u529b\u4e8e\u4e3a\u57fa\u56e0\u7ec4\u5b66\u7a81\u7834\u5f00\u8f9f\u65b0\u8def\u5f84\uff0c\u73b0\u5728\u6211\u4eec\u81f4\u529b\u4e8e\u52a0\u901f\u591a\u7ec4\u5b66\u5404\u4e2a\u9886\u57df\u7684\u8fdb\u5c55\u3002\u6211\u4eec\u7684\u6280\u672f\u5c06\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u66f4\u5168\u9762\u5730\u4e86\u89e3\u751f\u7269\u5b66\uff0c\u52a0\u5feb\u5c06\u6570\u636e\u8f6c\u5316\u4e3a\u6d1e\u5bdf\uff0c\u4ece\u800c\u771f\u6b63\u5b9e\u73b0\u7cbe\u51c6\u533b\u7597\u3002” \u56e0\u7f8e\u7eb3\u9996\u5e2d\u6280\u672f\u5b98Steve Barnard\u535a\u58eb\u8868\u793a\uff0c”\u56e0\u7f8e\u7eb3\u4e00\u5982\u65e2\u5f80\u5730\u63a8\u52a8\u5f00\u62d3\u6027\u521b\u65b0\u5e76\u5851\u9020\u884c\u4e1a\u6807\u6746\u3002\u6b63\u5982\u7814\u7a76\u4eba\u5458\u6240\u4fe1\u8d56\u7684\u51c6\u786e\u3001\u5b8c\u6574\u4e14\u5168\u9762\u7684\u57fa\u56e0\u7ec4\u89c6\u56fe\u4e00\u6837\uff0c\u56e0\u7f8e\u7eb3\u73b0\u5728\u5c06\u8fd9\u4e9b\u5c5e\u6027\u5f15\u5165\u6574\u4e2a\u591a\u7ec4\u5b66\u9886\u57df\u7684\u63a2\u7d22\u4e2d\uff0c\u5e76\u63d0\u4f9b\u4e86\u5145\u5206\u4fdd\u969c\u4ef7\u503c\u4e0e\u53ef\u7528\u6027\u7684\u5b8c\u6574\u5de5\u4f5c\u6d41\u7a0b\u3002\u8fd9\u4e9b\u89e3\u51b3\u65b9\u6848\u662f\u79d1\u7814\u53d1\u73b0\u7684\u4e00\u6b21\u98de\u8dc3\uff0c\u6211\u4eec\u975e\u5e38\u671f\u5f85\u5c06\u5176\u5e26\u5165\u66f4\u591a\u7684\u5b9e\u9a8c\u5ba4\u3002” \u56e0\u7f8e\u7eb3\u5728AGBT\u4e0a\u7684\u7cfb\u5217\u53d1\u5e03\u9a71\u52a8\u591a\u7ec4\u5b66\u53d1\u73b0\u89c4\u6a21\u4e0e\u8d28\u91cf\u63d0\u5347 \u5728AGBT\u7814\u8ba8\u4f1a\u4e0a\uff0c\u56e0\u7f8e\u7eb3\u5c06\u5c55\u793a\u57fa\u4e8e\u5176\u6d4b\u5e8f\u5e73\u53f0\u53ca\u5168\u65b0\u591a\u6a21\u6001\u6570\u636e\u5206\u6790\u5e73\u53f0\u7684\u591a\u7ec4\u5b66\u80fd\u529b\u7684\u6700\u65b0\u8fdb\u5c55\u3002\u8fd9\u4e9b\u8fdb\u5c55\u8868\u660e\uff0c\u56e0\u7f8e\u7eb3\u6301\u7eed\u81f4\u529b\u4e8e\u4e3a\u5ba2\u6237\u63d0\u4f9b\u7b80\u5316\u5de5\u4f5c\u6d41\u7a0b\u7684\u7aef\u5230\u7aef\u89e3\u51b3\u65b9\u6848\uff0c\u5e76\u4e0d\u65ad\u6269\u5927\u5176\u591a\u7ec4\u5b66\u4ea7\u54c1\u7ec4\u5408\uff0c\u5176\u4e2d\u5305\u62ec\u00a0Illumina Single Cell \u548c\u00a0Illumina Protein Prep\u3002 “\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6\u6280\u672f”\u89e3\u9501\u7f55\u89c1\u75c5\u68c0\u6d4b\u7684\u5168\u65b0\u53ef\u80fd\u3002\u56e0\u7f8e\u7eb3\u7684”\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6\uff08constellation mapped read\uff09”\u6280\u672f\u65e8\u5728\u52a0\u6df1\u5bf9\u57fa\u56e0\u7ec4\u7684\u7406\u89e3\uff0c\u5e76\u7b80\u5316\u5168\u57fa\u56e0\u7ec4\u5de5\u4f5c\u6d41\u7a0b\uff0c\u540c\u65f6\u51cf\u5c11\u4f20\u7edf\u7684\u6587\u5e93\u5236\u5907\u3002\u8be5\u6280\u672f\u76ee\u524d\u6b63\u5728\u5f00\u53d1\u4e2d\uff0c\u65e9\u671f\u6d4b\u8bd5\u8868\u660e\uff0c\u5176\u5728\u590d\u6742\u57fa\u56e0\u7ec4\u533a\u57df\u3001\u8d85\u957f\u76f8\u4f4d\u548c\u5927\u578b\u7ed3\u6784\u91cd\u6392\u65b9\u9762\u7684\u68c0\u6d4b\u80fd\u529b\u5f97\u5230\u4e86\u589e\u5f3a\u3002\u8fd9\u4e9b\u8fdb\u6b65\u6709\u671b\u4e3a\u66f4\u52a0\u5feb\u901f\u3001\u5168\u9762\u7684\u75be\u75c5\u68c0\u6d4b\u63d0\u4f9b\u652f\u6301\u3002 Rady\u513f\u7ae5\u57fa\u56e0\u7ec4\u533b\u5b66\u7814\u7a76\u6240\u603b\u88c1\u517c\u9996\u5e2d\u6267\u884c\u5b98Stephen Kingsmore\u535a\u58eb\u5c06\u5206\u4eab\u5176\u5bf9”\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6”\u6280\u672f\u7684\u7ecf\u9a8c\uff0c\u4ee5\u53ca\u8be5\u6280\u672f\u5bf9\u5168\u9762\u3001\u5feb\u901f\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u7684\u6f5c\u5728\u5f71\u54cd\uff0c\u4ece\u800c\u5b9e\u73b0\u66f4\u65e9\u7684\u9057\u4f20\u548c\u7f55\u89c1\u75be\u75c5\u8bca\u65ad\u3002 “\u2018\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6’\u6280\u672f\u80fd\u591f\u63d0\u4f9b\u7ed3\u6784\u6027\u53d8\u5f02\u7684\u9ad8\u5206\u8fa8\u7387\u89c6\u56fe\u2014\u2014\u5305\u62ec\u5bfc\u81f4\u7537\u5b69A\u578b\u8840\u53cb\u75c5\u7684\u6700\u5e38\u89c1\u539f\u56e0\uff0c\u8fd9\u662f\u6807\u51c6\u65b9\u6cd5\u76ee\u524d\u65e0\u6cd5\u5b9e\u73b0\u7684\u2014\u2014\u8fd9\u662f\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u9886\u57df\u4ee4\u4eba\u5370\u8c61\u6df1\u523b\u7684\u8fdb\u6b65\uff0c”\u00a0Kingsmore\u8868\u793a\uff0c”\u4ee5\u524d\uff0c\u8fd9\u4e9b\u53d8\u5f02\u5fc5\u987b\u901a\u8fc7\u5b9a\u5236\u7684\u4e00\u6b21\u6027\u6d4b\u5b9a\u6765\u786e\u5b9a\uff0c\u901a\u5e38\u9700\u8981\u4e00\u4e2a\u6708\u6216\u66f4\u957f\u65f6\u95f4\u3002\u2018\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6’\u6280\u672f\u8ba9\u6211\u4eec\u80fd\u591f\u5728\u4ec5\u4e00\u5929\u591a\u7684\u65f6\u95f4\u5185\u5b8c\u6210\u4ece\u5feb\u901f\u6d4b\u5e8f\u5230\u6df1\u5165\u5206\u6790\u7684\u8fc7\u7a0b\u3002” \u7f8e\u56fd\u4e1c\u90e8\u65f6\u95f42\u670824\u65e5\u661f\u671f\u4e00\u4e0b\u53484:30\u81f34:50\uff0cKingsmore\u535a\u58eb\u5c06\u5728AGBT\u5e76\u884c\u4f1a\u8bae\u4e0a\u53d1\u8868\u9898\u4e3a\u300a\u65b0\u578b\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u5de5\u4f5c\u6d41\u7a0b\u52a9\u529b\u8bbe\u8ba1\u9488\u5bf9\u7f55\u89c1\u9057\u4f20\u75c5\u60a3\u8005\u7684\u7b49\u4f4d\u57fa\u56e0\u7279\u5f02\u6027\u53cd\u4e49\u5be1\u6838\u82f7\u9178\u300b\u7684\u6458\u8981\u3002\u57fa\u4e8e”\u661f\u5ea7\u6620\u5c04\u8bfb\u53d6”\u6280\u672f\u7684\u4ea7\u54c1\u9884\u8ba1\u5c06\u4e8e2026\u5e74\u8fdb\u884c\u5546\u4e1a\u53d1\u5e03\u3002 \u56e0\u7f8e\u7eb3\u63a8\u51fa\u9762\u5411CRISPR\u7814\u7a76\u7684\u5355\u7ec6\u80de\u89e3\u51b3\u65b9\u6848\u3002\u5355\u7ec6\u80de\u6280\u672f\u6709\u671b\u4e3a\u80bf\u7624\u5b66\u3001\u514d\u75ab\u5b66\u548c\u836f\u7269\u9776\u70b9\u53d1\u73b0\u7b49\u5e7f\u6cdb\u7684\u7814\u7a76\u9886\u57df\u5e26\u6765\u91cd\u8981\u7684\u751f\u7269\u5b66\u89c1\u89e3\u3002\u5168\u65b0\u00a0Perturb-seq \u89e3\u51b3\u65b9\u6848\u4ee5\u524d\u6240\u672a\u6709\u7684\u89c4\u6a21\u4e3a\u56e0\u7f8e\u7eb3\u7684\u591a\u7ec4\u5b66\u4ea7\u54c1\u7ec4\u5408\u5e26\u6765\u4e86\u00a0Perturb-seq \u5b9e\u9a8c\u7684\u65b0\u529f\u80fd\uff0c\u4ece\u800c\u4f7f\u5f97\u5728\u5355\u7ec6\u80de\u5c42\u9762\u4e0a\u53d1\u73b0\u836f\u7269\u4f5c\u7528\u673a\u5236\uff0c\u5e76\u5bf9\u590d\u6742\u9057\u4f20\u75be\u75c5\u7684\u6cbb\u7597\u65b9\u6cd5\u8fdb\u884c\u4e34\u5e8a\u7814\u7a76\u6210\u4e3a\u53ef\u80fd\u3002\u8fd9\u9879\u6280\u672f\u8fd8\u5c06\u7528\u4e8e\u4e0a\u5468\u5ba3\u5e03\u7684\u4e0e\u535a\u5fb7\u4e34\u5e8a\u5b9e\u9a8c\u5ba4\uff08Broad Clinical Labs\uff09\u00a0\u7684\u5408\u4f5c\uff0c\u65e8\u5728\u5feb\u901f\u6269\u5c55\u5355\u7ec6\u80de\u89e3\u51b3\u65b9\u6848\uff0c\u5e76\u5728\u4e09\u5e74\u5185\u5b9e\u73b0\u00a050 \u4ebf\u5355\u7ec6\u80de\u56fe\u8c31\u751f\u6001\u7cfb\u7edf\u3002 \u8be5\u89e3\u51b3\u65b9\u6848\u9884\u8ba1\u5c06\u57282025\u5e74\u5e95\u63a8\u51fa\uff0c\u63d0\u4f9b\u4e09\u79cd\u65b0\u7684\u8bd5\u5242\u76d2\u578b\u53f7\uff0c\u5206\u522b\u53ef\u517c\u5bb9\u6bcf\u4efd\u6837\u672c10,000\u3001100,000\u548c1,000,000\u4e2a\u7ec6\u80de\uff0c\u4ee5\u652f\u6301\u4ece100\u4e2a\u5f15\u5bfcRNA\uff08gRNA\uff09\u5e8f\u5217\u7684\u9776\u5411\u7814\u7a76\u5230\u5355\u6b21\u5b9e\u9a8c\u8d85\u8fc710,000\u4e2agRNA\u7684\u5168\u57fa\u56e0\u7ec4\u89c4\u6a21\u7684\u53d1\u73b0\u3002\u8be5\u89e3\u51b3\u65b9\u6848\u5229\u7528\u5bf9\u00a0Fluent PIPseq \u57fa\u7840\u6280\u672f\u7684\u6700\u65b0\u6539\u8fdb\uff0c\u5728\u540c\u4e00\u68c0\u6d4b\u4e2d\u5bf9\u00a0CRISPR gRNA \u5e8f\u5217\u548c\u00a03′ RNA \u57fa\u56e0\u8868\u8fbe\u8fdb\u884c\u53cc\u91cd\u6d4b\u91cf\u3002\u4e0e\u76ee\u524d\u7684\u5355\u7ec6\u80de\u8bd5\u5242\u76d2\u76f8\u6bd4\uff0cgRNA 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\u56e0\u7f8e\u7eb3\u76845\u78b1\u57fa\u89e3\u51b3\u65b9\u6848\u7528\u4e8e\u7532\u57fa\u5316\u5206\u6790\u548c\u53d8\u5f02\u68c0\u6d4b\uff0c\u63d0\u5347\u4e86\u9057\u4f20\u53ca\u8868\u89c2\u9057\u4f20\u6570\u636e\u7684\u51c6\u786e\u6027\u3002\u56e0\u7f8e\u7eb3\u7684\u00a05 \u78b1\u57fa\u7532\u57fa\u5316\u5206\u6790\u89e3\u51b3\u65b9\u6848\u76ee\u524d\u5904\u4e8e\u65e9\u671f\u8bd5\u7528\u9636\u6bb5\uff0c\u53ef\u5728\u4e00\u6b21\u68c0\u6d4b\u4e2d\u540c\u65f6\u8fdb\u884c\u57fa\u56e0\u53d8\u5f02\u548c\u7532\u57fa\u5316\u68c0\u6d4b\u3002DNA \u7532\u57fa\u5316\u4f1a\u5f71\u54cd\u57fa\u56e0\u7684\u8868\u8fbe\u65b9\u5f0f\u548c\u65f6\u95f4\u3002\u68c0\u6d4b\u548c\u5206\u6790\u00a0DNA \u7532\u57fa\u5316\u7684\u80fd\u529b\u80fd\u591f\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u6df1\u5316\u5bf9\u00a0DNA 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\u56e0\u7f8e\u7eb3\u63a8\u51fa\u9762\u5411CRISPR\u7814\u7a76\u7684\u5355\u7ec6\u80de\u89e3\u51b3\u65b9\u6848\u3002\u5355\u7ec6\u80de\u6280\u672f\u6709\u671b\u4e3a\u80bf\u7624\u5b66\u3001\u514d\u75ab\u5b66\u548c\u836f\u7269\u9776\u70b9\u53d1\u73b0\u7b49\u5e7f\u6cdb\u7684\u7814\u7a76\u9886\u57df\u5e26\u6765\u91cd\u8981\u7684\u751f\u7269\u5b66\u89c1\u89e3\u3002\u5168\u65b0\u00a0Perturb-seq \u89e3\u51b3\u65b9\u6848\u4ee5\u524d\u6240\u672a\u6709\u7684\u89c4\u6a21\u4e3a\u56e0\u7f8e\u7eb3\u7684\u591a\u7ec4\u5b66\u4ea7\u54c1\u7ec4\u5408\u5e26\u6765\u4e86\u00a0Perturb-seq 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